Hotspot

rps4-trnT-UGU

LSC · 46664-47085 · 422 bp

Back to Sporoxeia

Pi 0.0166
MarkerSeek score 68.50
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
422
variable_sites
10
pi
0.0166
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAGACCTGGTAAAGCCCCAA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 542-551 bp 1.0 81.6
rps4-trnT-UGU_p2 TTAGACCTGGTAAAGCCCCA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 543-552 bp 1.0 81.6
rps4-trnT-UGU_p3 AAAATCGCTTCCGGCTCTAG CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 575-584 bp 1.0 81.4
rps4-trnT-UGU_p4 CTAAAATCGCTTCCGGCTCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 577-586 bp 1.0 81.4
rps4-trnT-UGU_p5 TCTAAAATCGCTTCCGGCTC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 578-587 bp 1.0 81.3