Hotspot

trnC-GCA-petN

LSC · 28452-29297 · 846 bp

Back to Sporoxeia

Pi 0.00946

MarkerSeek score 52.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 12
pi: 0.00946
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	902-915 bp	1.0	79.0
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	920-933 bp	1.0	76.8
trnC-GCA-petN_p3	ATTTTGGTTTGGCGACATGG	CAGCCCAAGCAAGACTTACT	45.0	50.0	57.5	57.8	954-967 bp	1.0	75.9
trnC-GCA-petN_p4	CCAGTTCAAATCCGGGTGTC	CAGCCCAAGCAAGACTTACT	55.0	50.0	58.8	57.8	897-910 bp	1.0	72.9
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCAGCCCAAGCAAGACTTA	50.0	52.6	58.1	58.1	903-916 bp	1.0	72.1