Hotspot

trnH-GUG-psbA

LSC · 76-306 · 231 bp

Back to Sporoxeia

Pi 0.0495
MarkerSeek score 73.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
231
variable_sites
17
pi
0.0495
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 285-287 bp 1.0 82.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 299-301 bp 1.0 82.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 298-300 bp 1.0 82.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 300-302 bp 1.0 82.2
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGCTGTTGAAGTTCCATC 55.0 50.0 58.1 57.4 284-286 bp 1.0 79.9