Hotspot

rpl32-trnL-UAG

SSC · 104413-104918 · 506 bp

Back to Sporobolus

Pi 0.0405

MarkerSeek score 78.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 506
variable_sites: 53
pi: 0.0405
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAGCCAAAACCAAAAGGTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	593-615 bp	1.0	90.5
rpl32-trnL-UAG_p2	GCAGCCAAAACCAAAAGGTT	ATGTCCCGAAGAAACCCAAC	45.0	50.0	58.0	58.1	906-941 bp	1.0	88.2
rpl32-trnL-UAG_p3	GCAGCCAAAACCAAAAGGTT	CCGAAGAAACCCAACGAGAA	45.0	50.0	58.0	58.1	901-936 bp	1.0	88.0
rpl32-trnL-UAG_p4	GCAGCCAAAACCAAAAGGTT	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.0	58.0	1172-1207 bp	1.0	87.4
rpl32-trnL-UAG_p5	GAGCAGCCAAAACCAAAAGG	GATCCGCACAAAAGAGTTGC	50.0	50.0	58.1	58.0	1174-1209 bp	1.0	86.7