Hotspot

rpl33-rps18

LSC · 64919-65195 · 277 bp

Back to Sporobolus

Pi 0.0348

MarkerSeek score 71.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 25
pi: 0.0348
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAAGGAGGTTCATGGCCAAG	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.1	58.0	833-866 bp	0.888889	79.8
rpl33-rps18_p2	GCCTCACAAATCCGAGCATA	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	1051-1063 bp	0.666667	70.3
rpl33-rps18_p3	GCCTCACAAATCCGAGCATA	TTTCTGTGTCTAGGACCGGT	50.0	50.0	58.0	58.0	1083-1095 bp	0.666667	70.2
rpl33-rps18_p4	GCCTCACAAATCCGAGCATA	GTAGGAATCAACGAGCGTGT	50.0	50.0	58.0	58.0	1332-1391 bp	0.666667	70.1
rpl33-rps18_p5	AAAGGAGGTTCATGGCCAAG	TTTCTGTGTCTAGGACCGGT	50.0	50.0	58.1	58.0	865-874 bp	0.666667	69.2