Hotspot

rbcL-psaI

LSC · 55802-56685 · 884 bp

Back to Sporobolus

Pi 0.0315
MarkerSeek score 73.30
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
884
variable_sites
68
pi
0.0315
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 GATCATATCCACGCCGGTAC CGCGATTCCGTAGAAAGACA 55.0 50.0 58.0 58.1 1986-2048 bp 1.0 87.9
rbcL-psaI_p2 GATCATATCCACGCCGGTAC GGAGCTAATGAATAGGCCCG 55.0 55.0 58.0 57.9 2004-2066 bp 1.0 87.8
rbcL-psaI_p3 AAGCCGGTAGATACCATCGA CGCGATTCCGTAGAAAGACA 50.0 50.0 58.0 58.1 1577-1610 bp 0.666667 73.6
rbcL-psaI_p4 GATCATATCCACGCCGGTAC TTGCAATTGCCGGAACTACT 55.0 45.0 58.0 58.1 1413 bp 0.111111 43.6
rbcL-psaI_p5 AAGCCGGTAGATACCATCGA TTGCAATTGCCGGAACTACT 50.0 45.0 58.0 58.1 975 bp 0.111111 43.6