Pi
0.0328
MarkerSeek score
73.00
Primer available
yes
Label
ndhC-trnV-UAC
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhC-trnV-UAC_p1 | TCATTGCCCAAGGGTAGAGA | GGTTCGAATCCGTATAGCCC | 50.0 | 55.0 | 58.0 | 57.9 | 757-1193 bp | 1.0 | 88.9 |
| ndhC-trnV-UAC_p2 | TCATTGCCCAAGGGTAGAGA | ATAGTGAACTCTTGCAGCGG | 50.0 | 50.0 | 58.0 | 58.0 | 1079-1515 bp | 1.0 | 88.3 |
| ndhC-trnV-UAC_p3 | AAAACTCATTGCCCAAGGGT | ATAGTGAACTCTTGCAGCGG | 45.0 | 50.0 | 57.9 | 58.0 | 1084-1520 bp | 1.0 | 87.8 |
| ndhC-trnV-UAC_p4 | TGCCCAAGGGTAGAGAAAGA | GGTTCGAATCCGTATAGCCC | 50.0 | 55.0 | 58.0 | 57.9 | 1100-1189 bp | 0.777778 | 78.6 |
| ndhC-trnV-UAC_p5 | TGCCCAAGGGTAGAGAAAGA | ATAGTGAACTCTTGCAGCGG | 50.0 | 50.0 | 58.0 | 58.0 | 1422-1511 bp | 0.777778 | 78.2 |