Hotspot

trnF-GAA-ndhJ

LSC · 47557-48087 · 531 bp

Back to Sporobolus

Pi 0.036

MarkerSeek score 75.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 47
pi: 0.036
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	586-595 bp	1.0	48.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.4	587-596 bp	1.0	48.3
trnF-GAA-ndhJ_p3	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.5	53.8	589-598 bp	1.0	48.2
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	639-648 bp	1.0	47.6
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.8	53.8	637-646 bp	1.0	47.6