Hotspot

trnC-GCA-rpoB

LSC · 17901-19065 · 1165 bp

Back to Sporobolus

Pi 0.0301

MarkerSeek score 69.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 95
pi: 0.0301
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAGACGCGGGAATAGAGAGA	CCGCCTACACAAGCAAATTG	50.0	50.0	57.9	58.0	2510-2610 bp	1.0	86.1
trnC-GCA-rpoB_p2	AGAAGACGCGGGAATAGAGA	CCGCCTACACAAGCAAATTG	50.0	50.0	57.9	58.0	2512-2612 bp	1.0	86.1
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGCCTACACAAGCAAATTG	50.0	50.0	58.1	58.0	1893-1907 bp	1.0	84.8
trnC-GCA-rpoB_p4	TGCCCCAAATTAGGAGGGTA	CCGCCTACACAAGCAAATTG	50.0	50.0	58.0	58.0	1998-2004 bp	0.333333	54.1
trnC-GCA-rpoB_p5	CGCCAAAACAAAACCTCGTT	CCGCCTACACAAGCAAATTG	45.0	50.0	58.1	58.0	2076-2080 bp	0.222222	48.9