Hotspot

petN-trnC-GCA

LSC · 17008-17829 · 822 bp

Back to Sporobolus

Pi 0.0291
MarkerSeek score 71.80
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
822
variable_sites
67
pi
0.0291
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTTCTCCCCCATAC AAAGGAATGGAGGGGGAGAA 55.0 50.0 58.1 57.9 1344-1462 bp 0.777778 75.0
petN-trnC-GCA_p2 GTCCACTTCTCCCCCATACT AAAGGAATGGAGGGGGAGAA 55.0 50.0 58.1 57.9 1343-1461 bp 0.777778 75.0
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA AAAGGAATGGAGGGGGAGAA 50.0 50.0 58.2 57.9 1285-1403 bp 0.777778 74.9
petN-trnC-GCA_p4 AGTCCACTTCTCCCCCATAC AAAAGGAATGGAGGGGGAGA 55.0 50.0 58.1 57.9 1349-1463 bp 0.222222 49.8
petN-trnC-GCA_p5 GTCCACTTCTCCCCCATACT AAAAGGAATGGAGGGGGAGA 55.0 50.0 58.1 57.9 1348-1462 bp 0.222222 49.8