Hotspot

trnG-UCC-trnT-GGU

LSC · 13343-13931 · 589 bp

Back to Sporobolus

Pi 0.0327
MarkerSeek score 75.30
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
589
variable_sites
51
pi
0.0327
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 679-1109 bp 1.0 45.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 675-1105 bp 1.0 44.9
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 676-1106 bp 1.0 44.9
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 682-1112 bp 1.0 44.9
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 681-1111 bp 1.0 44.9