Hotspot

trnK-UUU-rps16

LSC · 3930-4490 · 561 bp

Back to Sporobolus

Pi 0.0286
MarkerSeek score 70.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
561
variable_sites
43
pi
0.0286
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCCCCGTATTAGGCAC TCGAGCCGTATGAGGAGAAA 55.0 50.0 58.0 58.2 1076-1199 bp 1.0 86.3
trnK-UUU-rps16_p2 CCTTTCCCCGTATTAGGCAC TTTCTCGAGCCGTATGAGGA 55.0 50.0 58.0 58.2 1080-1203 bp 1.0 86.3
trnK-UUU-rps16_p3 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.1 58.2 866-975 bp 1.0 86.2
trnK-UUU-rps16_p4 TGAGGATCAGTCGTGGTCTT TTTCTCGAGCCGTATGAGGA 50.0 50.0 58.1 58.2 870-979 bp 1.0 86.2
trnK-UUU-rps16_p5 CCTTTCCCCGTATTAGGCAC GCTCAACCCACAAGAACTGT 55.0 50.0 58.0 58.3 865-987 bp 1.0 85.9