Hotspot

ycf1

SSC · 131104-136545 · 5442 bp

Back to Spondias

Pi 0.00975

MarkerSeek score 45.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5442
variable_sites: 99
pi: 0.00975
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAGGAACTTTTTGACTAATTTCTCT	AGACAATACACGAATTGAGT	34.6	35.0	57.9	52.3	205 bp	1.0	44.7
ycf1_p2	CGAGGAACTTTTTGACTAATTTCTC	AGACAATACACGAATTGAGT	36.0	35.0	56.7	52.3	205 bp	1.0	44.7
ycf1_p3	CGAGGAACTTTTTGACTAATTTCT	AGACAATACACGAATTGAGT	33.3	35.0	55.7	52.3	205 bp	1.0	44.7
ycf1_p4	CGAGGAACTTTTTGACTAATTTC	AGACAATACACGAATTGAGT	34.8	35.0	54.3	52.3	205 bp	1.0	44.7
ycf1_p5	CGAGGAACTTTTTGACTAATT	AGACAATACACGAATTGAGT	33.3	35.0	52.1	52.3	205 bp	1.0	44.7