Hotspot

ccsA-ndhD

SSC · 122488-122801 · 314 bp

Back to Spondias

Pi 0.0292

MarkerSeek score 67.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 314
variable_sites: 18
pi: 0.0292
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCTATTTCGGGGTCAATCT	ATCCGGATTTCGTTCTCTCC	45.0	50.0	56.6	57.1	445-457 bp	1.0	65.3
ccsA-ndhD_p2	TCGGGGTCAATCTAGTAGGA	ATCCGGATTTCGTTCTCTCC	50.0	50.0	56.6	57.1	438-450 bp	1.0	65.0
ccsA-ndhD_p3	GCAATTGTAGCTTCTATGGGC	ATCCGGATTTCGTTCTCTCC	47.6	50.0	57.4	57.1	484-496 bp	1.0	63.1
ccsA-ndhD_p4	TGCTATTTCGGGGTCAATCT	CGTTCTCTCCCTATCAGTGG	45.0	55.0	56.6	56.8	435-447 bp	1.0	63.1
ccsA-ndhD_p5	TCGGGGTCAATCTAGTAGGAA	ATCCGGATTTCGTTCTCTCC	47.6	50.0	57.3	57.1	438-450 bp	1.0	63.1