Hotspot

ndhF-rpl32

SSC · 118995-119928 · 934 bp

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Pi 0.0129

MarkerSeek score 60.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 934
variable_sites: 21
pi: 0.0129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	CCAATATCCCCTGGCTTTCC	45.0	55.0	58.0	58.0	1108-1206 bp	1.0	84.8
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	CCCAATATCCCCTGGCTTTC	45.0	55.0	58.0	58.0	1109-1207 bp	1.0	84.8
ndhF-rpl32_p3	AGGTCGGGTAAACCAAAACC	CCAATATCCCCTGGCTTTCC	50.0	55.0	58.0	58.0	1516-1614 bp	1.0	84.1
ndhF-rpl32_p4	AGGTCGGGTAAACCAAAACC	CCCAATATCCCCTGGCTTTC	50.0	55.0	58.0	58.0	1517-1615 bp	1.0	84.1
ndhF-rpl32_p5	AGGTCGGGTAAACCAAAACC	TTCGAGTCATGCGGATTCAG	50.0	50.0	58.0	58.1	1633-1731 bp	0.75	70.6