Hotspot

trnR-ACG-trnN-GUU

SSC · 114332-114935 · 604 bp

Back to Spondias

Pi 0.00524

MarkerSeek score 62.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 604
variable_sites: 6
pi: 0.00524
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	TAGGGTACTTCCGGGAACAG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.1	58.1	837-843 bp	1.0	78.7
trnR-ACG-trnN-GUU_p2	TAGGGTACTTCCGGGAACAG	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.1	57.8	760-766 bp	1.0	78.3
trnR-ACG-trnN-GUU_p3	TAGGGTACTTCCGGGAACAG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.1	58.2	747-753 bp	1.0	78.2
trnR-ACG-trnN-GUU_p4	TAGGGTACTTCCGGGAACAG	TAGAATCTCCCGCTGTTCCT	55.0	50.0	58.1	57.8	805-811 bp	1.0	78.2
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	786-792 bp	1.0	78.1