Hotspot

petA-psbJ

LSC · 68173-69255 · 1083 bp

Back to Spondias

Pi 0.0246

MarkerSeek score 66.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 48
pi: 0.0246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTGGCATCGGTTGTTTTGG	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	1193-1205 bp	1.0	77.4
petA-psbJ_p2	TTTGGCATCGGTTGTTTTGG	GGATTGGGTTCGTCCTTGTA	45.0	50.0	57.8	57.2	1187-1199 bp	1.0	76.1
petA-psbJ_p3	ACGTATCCAAGGCCTTTTGT	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.4	57.4	1217-1229 bp	1.0	74.5
petA-psbJ_p4	TTTGGCATCGGTTGTTTTGG	TCATATTCCGGATTGGGTTCG	45.0	47.6	57.8	57.9	1196-1208 bp	1.0	73.3
petA-psbJ_p5	TTTGGCATCGGTTGTTTTGG	ATATTCCGGATTGGGTTCGTC	45.0	47.6	57.8	57.9	1194-1206 bp	1.0	73.2