Hotspot

accD-psaI

LSC · 63649-64274 · 626 bp

Back to Spondias

Pi 0.0197

MarkerSeek score 58.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 24
pi: 0.0197
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCGAGAAAATCACCCGTTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1154-1164 bp	1.0	81.6
accD-psaI_p2	CGGTTGATCCAGGTACTTGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1382-1392 bp	1.0	81.3
accD-psaI_p3	GCGAGAAAATCACCCGTTTG	AATAAGAACAGCCCCCTCCT	50.0	50.0	58.0	58.0	1469-1484 bp	0.75	71.6
accD-psaI_p4	CGGTTGATCCAGGTACTTGG	AATAAGAACAGCCCCCTCCT	55.0	50.0	58.0	58.0	1697-1712 bp	0.75	71.4
accD-psaI_p5	ACCCGTTTGATCGAGTATGC	AATAAGAACAGCCCCCTCCT	50.0	50.0	58.1	58.0	1458-1473 bp	0.75	71.3