Hotspot

ndhC-trnT-GGU

LSC · 55274-57125 · 1852 bp

Back to Spondias

Pi 0.0136
MarkerSeek score 58.40
Primer available yes
Label ndhC-trnT-GGU

Feature Metadata

label_name
ndhC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1852
variable_sites
45
pi
0.0136
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnT-GGU_p1 TCATATTTGTAAAGCAGAAACA CCCGCCGTATGAAAGCAATA 27.3 50.0 52.0 58.4 1847-1913 bp 1.0 45.0
ndhC-trnT-GGU_p2 TCATATTTGTAAAGCAGAAACA CCGCCGTATGAAAGCAATAC 27.3 50.0 52.0 57.3 1846-1912 bp 1.0 45.0
ndhC-trnT-GGU_p3 ATCATATTTGTAAAGCAGAAACA CCCGCCGTATGAAAGCAATA 26.1 50.0 52.7 58.4 1848-1914 bp 1.0 45.0
ndhC-trnT-GGU_p4 TCATATTTGTAAAGCAGAAACAT CCCGCCGTATGAAAGCAATA 26.1 50.0 52.7 58.4 1847-1913 bp 1.0 45.0
ndhC-trnT-GGU_p5 TCATATTTGTAAAGCAGAAACA TTTGAACCAATGACTCCCGC 27.3 50.0 52.0 58.8 1862-1928 bp 1.0 45.0