Hotspot

atpF-atpH

LSC · 14986-15496 · 511 bp

Back to Spondias

Pi 0.0101

MarkerSeek score 60.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 9
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGAACCAAGGAAACGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	666-710 bp	1.0	75.5
atpF-atpH_p2	CCAGTGAACCAAGGAAACGA	CGAGGCGGAGGGAAAAATAC	50.0	55.0	58.0	58.7	671-715 bp	1.0	75.4
atpF-atpH_p3	CCAGTGAACCAAGGAAACGA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.0	58.7	670-714 bp	1.0	75.4
atpF-atpH_p4	CCAGTGAACCAAGGAAACGA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	667-711 bp	1.0	75.4
atpF-atpH_p5	CCAGTGAACCAAGGAAACGA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	668-712 bp	1.0	75.4