Hotspot

trnS-GCU-trnR-UCU

LSC · 9935-11854 · 1920 bp

Back to Spondias

Pi 0.0141
MarkerSeek score 59.00
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1920
variable_sites
50
pi
0.0141
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CCGACGCTTTAATCCACTCA TGTCCTATCCATTAGACAATG 50.0 38.1 58.0 52.4 1961-1978 bp 1.0 45.5
trnS-GCU-trnR-UCU_p2 ACGCTTTAATCCACTCAGCC TGTCCTATCCATTAGACAATG 50.0 38.1 58.3 52.4 1958-1975 bp 1.0 45.5
trnS-GCU-trnR-UCU_p3 CGCTTTAATCCACTCAGCCA TGTCCTATCCATTAGACAATG 50.0 38.1 58.3 52.4 1957-1974 bp 1.0 45.5
trnS-GCU-trnR-UCU_p4 TCCGACGCTTTAATCCACTC TGTCCTATCCATTAGACAATG 50.0 38.1 57.7 52.4 1962-1979 bp 1.0 45.5
trnS-GCU-trnR-UCU_p5 GCAATCCGACGCTTTAATCC TGTCCTATCCATTAGACAATG 50.0 38.1 57.6 52.4 1966-1983 bp 1.0 45.5