Hotspot

trnK-UUU-rps16

LSC · 4674-5760 · 1087 bp

Back to Spondias

Pi 0.0146

MarkerSeek score 65.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 29
pi: 0.0146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1080-1187 bp	1.0	83.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1079-1186 bp	1.0	83.6
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.3	57.9	1089-1196 bp	1.0	82.6
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	1073-1180 bp	1.0	81.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	1072-1179 bp	1.0	81.7