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rps2

LSC · 49992-50702 · 711 bp

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Pi 0.00281

MarkerSeek score 39.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 4
pi: 0.00281
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TATATCATTCGTGGGCGCTG	CGTTCCCCACAAGACAAGAA	50.0	50.0	57.9	58.0	1842-1846 bp	1.0	80.0
rps2_p2	GGATGGTTTGTGGATTCCGA	CGTTCCCCACAAGACAAGAA	50.0	50.0	57.9	58.0	1633-1637 bp	1.0	79.8
rps2_p3	TCGGGATGGTTTGTGGATTC	CGTTCCCCACAAGACAAGAA	50.0	50.0	57.9	58.0	1636-1640 bp	1.0	79.8
rps2_p4	TATATCATTCGTGGGCGCTG	CATCGTTCCCCACAAGACAA	50.0	50.0	57.9	58.1	1845-1849 bp	1.0	79.4
rps2_p5	GTCGGGATGGTTTGTGGATT	CGTTCCCCACAAGACAAGAA	50.0	50.0	58.2	58.0	1637-1641 bp	1.0	79.4