Hotspot

rps16

LSC · 75668-76773 · 1106 bp

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Pi 0.00579

MarkerSeek score 56.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1106
variable_sites: 12
pi: 0.00579
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCTCGATACCATTTTGCAATGA	GGTACTAAGGACCCTCTCC	36.4	57.9	56.2	55.2	1179-1189 bp	1.0	41.5
rps16_p2	TCTCGATACCATTTTGCAATGA	TGGCACGATATAAATGTAGCA	36.4	38.1	56.2	55.2	1249-1259 bp	1.0	41.4
rps16_p3	TCTCCATAGATAGCAGGTTTGA	GGTACTAAGGACCCTCTCC	40.9	57.9	56.1	55.2	1253-1263 bp	1.0	41.4
rps16_p4	TCTCGATACCATTTTGCAATGA	AGAAAGAGGCAAATTGTGTC	36.4	40.0	56.2	54.1	1212-1222 bp	1.0	41.4
rps16_p5	TCTCCATAGATAGCAGGTTTGA	TGGCACGATATAAATGTAGCA	40.9	38.1	56.1	55.2	1323-1333 bp	1.0	41.3