Hotspot

ndhF

SSC · 114344-116560 · 2217 bp

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Pi 0.00577

MarkerSeek score 40.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 23
pi: 0.00577
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGTTTGGTGAATCGGAAACA	TGCATTTTGATGAGCCTCGT	42.9	45.0	58.1	58.2	2342-2347 bp	1.0	72.0
ndhF_p2	AGGTTTGGTGAATCGGAAACA	GTTTGCTGGCTTATTTGGCA	42.9	45.0	58.1	57.5	2480-2607 bp	1.0	70.5
ndhF_p3	AGGTTTGGTGAATCGGAAACA	ACGGAGACTTGCTTCACATT	42.9	45.0	58.1	57.4	2566-2693 bp	1.0	69.6
ndhF_p4	GGTTTGGTGAATCGGAAACA	TGCATTTTGATGAGCCTCGT	45.0	45.0	56.5	58.2	2341-2346 bp	1.0	68.8
ndhF_p5	AGGTTTGGTGAATCGGAAACA	CGTGAAATTCTCGAGCCCAA	42.9	50.0	58.1	58.6	2999 bp	0.4	43.0