Hotspot

trnL-UAG-rpl32

SSC · 112793-113323 · 531 bp

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Pi 0.00866
MarkerSeek score 42.70
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
531
variable_sites
11
pi
0.00866
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TTCGAACCGAGATGCTTGAG AGAAGGATTGATTTGCGGCA 50.0 45.0 58.0 58.2 844-850 bp 1.0 80.4
trnL-UAG-rpl32_p2 GGATTCGAACCGAGATGCTT AGAAGGATTGATTTGCGGCA 50.0 45.0 58.1 58.2 847-853 bp 1.0 79.9
trnL-UAG-rpl32_p3 GATCCGCACAAAAGAGTTGC AGAAGGATTGATTTGCGGCA 50.0 45.0 58.0 58.2 1396-1402 bp 1.0 79.6
trnL-UAG-rpl32_p4 TCCTAAGAGCAGCGTGTCTA AGAAGGATTGATTTGCGGCA 50.0 45.0 57.9 58.2 817-823 bp 1.0 79.5
trnL-UAG-rpl32_p5 ATGTCCCGAAGAAACCCAAC AGAAGGATTGATTTGCGGCA 50.0 45.0 58.1 58.2 1130-1136 bp 1.0 79.3