Hotspot

trnT-GGU-trnfM-CAU

LSC · 65813-68660 · 2848 bp

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Pi 0.00576

MarkerSeek score 51.70

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 2848
variable_sites: 29
pi: 0.00576
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	ACCACTAAGTGAAAAGCCCT	CCTCAAGGTTATGAGCCTCG	45.0	55.0	56.7	57.8	2975-2982 bp	1.0	68.3
trnT-GGU-trnfM-CAU_p2	TGAACCGATGACTTATGCCT	CCTCAAGGTTATGAGCCTCG	45.0	55.0	56.6	57.8	2945-2952 bp	1.0	68.1
trnT-GGU-trnfM-CAU_p3	ACCACTAAGTGAAAAGCCCT	CCTCGTGAGCTACCAAACTG	45.0	55.0	56.7	58.3	2960-2967 bp	1.0	67.8
trnT-GGU-trnfM-CAU_p4	TGAACCGATGACTTATGCCT	CCTCGTGAGCTACCAAACTG	45.0	55.0	56.6	58.3	2930-2937 bp	1.0	67.7
trnT-GGU-trnfM-CAU_p5	GACTTCTGTCTTACCAAGGCA	CCTCGTGAGCTACCAAACTG	47.6	55.0	57.6	58.3	2988-2995 bp	1.0	67.4