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atpH-atpI

LSC · 48177-48997 · 821 bp

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Pi 0.00268

MarkerSeek score 53.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 4
pi: 0.00268
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGTACCTTGACCAACTCCG	TAGCCGCAGCCTATATAGGT	55.0	50.0	57.9	57.5	954-957 bp	1.0	75.7
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	ATTTTTGCAACGTTAGCCGC	55.0	45.0	57.9	58.6	967-970 bp	1.0	75.3
atpH-atpI_p3	GCAGTACCTTGACCAACTCC	TAGCCGCAGCCTATATAGGT	55.0	50.0	58.2	57.5	955-958 bp	1.0	74.8
atpH-atpI_p4	AAGCAGCAGCAATTAGTGGA	TAGCCGCAGCCTATATAGGT	45.0	50.0	57.8	57.5	888-891 bp	1.0	74.8
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TAGCCGCAGCCTATATAGGT	50.0	50.0	57.8	57.5	910-913 bp	1.0	74.7