Hotspot

trnfM-CAU-trnR-UCU

LSC · 44086-44250 · 165 bp

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Pi 0.0255

MarkerSeek score 57.80

Primer available yes

Label trnfM-CAU-trnR-UCU

Feature Metadata

label_name: trnfM-CAU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 7
pi: 0.0255
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnR-UCU_p1	TCCCCGCTAAGAAGGTAGAG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.9	56.9	269-275 bp	1.0	65.1
trnfM-CAU-trnR-UCU_p2	CCAACCCCCTTTTATCCACC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	292-298 bp	1.0	64.4
trnfM-CAU-trnR-UCU_p3	TCCCCGCTAAGAAGGTAGAG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	57.9	57.9	275-281 bp	1.0	64.4
trnfM-CAU-trnR-UCU_p4	CCAACCCCCTTTTATCCACC	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	298-304 bp	1.0	63.7
trnfM-CAU-trnR-UCU_p5	ACCCCCTTTTATCCACCAGT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	289-295 bp	1.0	63.7