Hotspot

rpl16

LSC · 81387-82749 · 1363 bp

Back to Spiraea

Pi 0.00747

MarkerSeek score 46.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1363
variable_sites: 45
pi: 0.00747
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCGCTGTTATCCGCTACATT	CACCCCAATATTCTTGCGGA	50.0	50.0	58.1	57.9	1989-2005 bp	1.0	81.8
rpl16_p2	AACTGTCTTAATTCCCGGGC	CACCCCAATATTCTTGCGGA	50.0	50.0	57.9	57.9	2271-2287 bp	1.0	81.7
rpl16_p3	CCGCTGTTATCCGCTACATT	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.1	58.1	2187-2203 bp	1.0	81.5
rpl16_p4	AACTGTCTTAATTCCCGGGC	GTCGAGGGAATTGCACGTAT	50.0	50.0	57.9	58.1	2469-2485 bp	1.0	81.4
rpl16_p5	AACTGTCTTAATTCCCGGGC	TGCACGTGTTGAATGGATCA	50.0	45.0	57.9	58.1	2119-2135 bp	1.0	81.3