Hotspot

ndhF

SSC · 110696-112927 · 2232 bp

Back to Spiraea

Pi 0.00673

MarkerSeek score 40.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 70
pi: 0.00673
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	ACCGAACCCCTTTCAAAAGA	50.0	45.0	58.0	57.3	2545-2572 bp	1.0	76.9
ndhF_p2	GCAGAATACCGTCACCCATT	ACCGAACCCCTTTCAAAAGA	50.0	45.0	58.0	57.3	2655-2682 bp	1.0	76.9
ndhF_p3	AGAAGATCCTTCCCCTTCCC	ACCGAACCCCTTTCAAAAGA	55.0	45.0	58.1	57.3	2507-2534 bp	1.0	76.2
ndhF_p4	GAAGATCCTTCCCCTTCCCT	ACCGAACCCCTTTCAAAAGA	55.0	45.0	58.1	57.3	2506-2533 bp	1.0	76.2
ndhF_p5	GGAACAAGAGGGATTCACCG	ACCGAACCCCTTTCAAAAGA	55.0	45.0	58.3	57.3	2528-2555 bp	1.0	74.9