Hotspot

rpl32-trnL-UAG

SSC · 114152-115274 · 1123 bp

Back to Spiraea

Pi 0.0125
MarkerSeek score 57.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1123
variable_sites
67
pi
0.0125
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATCGGGCAGCATTGAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1023-1245 bp 1.0 87.7
rpl32-trnL-UAG_p2 GGATATCGGGCAGCATTGAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1024-1246 bp 1.0 87.6
rpl32-trnL-UAG_p3 GGATATCGGGCAGCATTGAA ATCCACACAAAAGAGCTGCA 50.0 45.0 58.1 58.0 1623-1844 bp 1.0 86.9
rpl32-trnL-UAG_p4 GGATATCGGGCAGCATTGAA TCCACACAAAAGAGCTGCAT 50.0 45.0 58.1 58.0 1622-1843 bp 1.0 86.9
rpl32-trnL-UAG_p5 GGATATCGGGCAGCATTGAA ATAACACTTGGCGCGGTTAT 50.0 45.0 58.1 58.0 1483-1704 bp 1.0 86.9