Hotspot

psbL-psbF

LSC · 65042-65063 · 22 bp

Back to Spiraea

Pi 0.0215

MarkerSeek score 54.80

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 2
pi: 0.0215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	ACGGACTAGCTGTACCTACC	45.0	55.0	58.0	58.0	113 bp	1.0	80.7
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	GGACTAGCTGTACCTACCGT	45.0	55.0	58.0	58.0	111 bp	1.0	80.7
psbL-psbF_p3	TCGTTCGGGTTTGATTGTGT	ATGGTTAGCTGTTCACGGAC	45.0	50.0	58.0	57.9	127 bp	1.0	80.2
psbL-psbF_p4	CAACACTTTGTTCGTTCGGG	ACGGACTAGCTGTACCTACC	50.0	55.0	57.9	58.0	124 bp	1.0	80.1
psbL-psbF_p5	CAACACTTTGTTCGTTCGGG	GGACTAGCTGTACCTACCGT	50.0	55.0	57.9	58.0	122 bp	1.0	80.1