Hotspot

trnF-GAA-ndhJ

LSC · 49928-50434 · 507 bp

Back to Spiraea

Pi 0.00585

MarkerSeek score 55.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 17
pi: 0.00585
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	674-701 bp	1.0	84.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	638-665 bp	1.0	84.3
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	662-689 bp	1.0	83.4
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	687-714 bp	1.0	83.2
trnF-GAA-ndhJ_p5	GTGAATGGTCGGGATAGCTC	ATCCACGCCTGAAACGTATC	55.0	50.0	57.8	58.1	684-711 bp	1.0	83.1