Hotspot

trnT-GGU-psbD

LSC · 32718-34038 · 1321 bp

Back to Spiraea

Pi 0.00862

MarkerSeek score 58.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1321
variable_sites: 55
pi: 0.00862
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1474-1522 bp	1.0	84.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1477-1525 bp	1.0	84.7
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1476-1524 bp	1.0	84.7
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1479-1527 bp	1.0	84.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GAAACGATCCCTCCGTAACC	55.0	55.0	58.1	58.1	1452-1500 bp	1.0	84.6