Hotspot

trnE-UUC-trnT-GGU

LSC · 32144-32646 · 503 bp

Back to Spiraea

Pi 0.00465

MarkerSeek score 54.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 14
pi: 0.00465
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	572-602 bp	1.0	84.7
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	570-600 bp	1.0	84.7
trnE-UUC-trnT-GGU_p3	AACTTCCTTTCGTAGCACCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	633-663 bp	1.0	84.4
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	551-581 bp	1.0	84.3
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	549-579 bp	1.0	84.3