Hotspot

petN-psbM

LSC · 29532-30127 · 596 bp

Back to Spiraea

Pi 0.018

MarkerSeek score 58.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 53
pi: 0.018
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	GTGGAAAGGTGGTTCGATGA	45.0	50.0	58.0	57.8	1111-1142 bp	1.0	87.2
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	AGTGGAAAGGTGGTTCGATG	45.0	50.0	58.0	57.8	1112-1143 bp	1.0	87.1
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	GGTTGGGTGGGGTTTGTTTA	45.0	50.0	58.0	58.2	1144-1175 bp	1.0	86.8
petN-psbM_p4	ATCTTGCTTGGGCTGCTTTA	GTGGAAAGGTGGTTCGATGA	45.0	50.0	57.8	57.8	1112-1143 bp	1.0	85.5
petN-psbM_p5	TAAATCTTGCTTGGGCTGCT	GTGGAAAGGTGGTTCGATGA	45.0	50.0	57.8	57.8	1115-1146 bp	1.0	85.4