Hotspot

atpF-atpH

LSC · 13460-13914 · 455 bp

Back to Spiraea

Pi 0.00475

MarkerSeek score 56.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 18
pi: 0.00475
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	620-637 bp	1.0	79.7
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	624-641 bp	1.0	79.5
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	621-638 bp	1.0	79.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	622-639 bp	1.0	79.5
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CAGAGGCGGAGGGAAAAATA	50.0	50.0	57.9	57.3	626-643 bp	1.0	79.4