Hotspot

rps16-trnQ-UUG

LSC · 6381-7226 · 846 bp

Back to Spiraea

Pi 0.00851

MarkerSeek score 61.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 42
pi: 0.00851
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	875-925 bp	1.0	86.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	886-936 bp	1.0	85.4
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	896-946 bp	1.0	83.7
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GTGTCGACTAGAAATGGGGC	45.0	55.0	58.1	58.3	937-987 bp	1.0	83.6
rps16-trnQ-UUG_p5	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	897-947 bp	1.0	83.6