Hotspot

trnH-GUG-psbA

LSC · 136-386 · 251 bp

Back to Spiraea

Pi 0.0237
MarkerSeek score 58.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
251
variable_sites
30
pi
0.0237
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 306-342 bp 1.0 86.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 320-356 bp 1.0 85.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 319-355 bp 1.0 85.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 321-357 bp 1.0 85.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGCTGTTGAAGTTCCATC 55.0 50.0 58.1 57.4 305-341 bp 1.0 83.6