Hotspot

accD

LSC · 56971-58476 · 1506 bp

Back to Sphenostylis

Pi 0.00465

MarkerSeek score 46.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1506
variable_sites: 7
pi: 0.00465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAAAAGGGGGTGTGGATGAC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2039-2230 bp	1.0	80.2
accD_p2	AAAAAGGGGGTGTGGATGAC	GTGGCCCCTTGTGGAATAAA	50.0	50.0	58.0	58.1	2448-2639 bp	1.0	79.8
accD_p3	GAGGTTCGAATCCTTCCGTC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2529-2716 bp	1.0	79.7
accD_p4	AAAAAGGGGGTGTGGATGAC	TGCAACCAACAATAACCCCA	50.0	45.0	58.0	57.9	2341-2532 bp	1.0	79.6
accD_p5	AAAAAGGGGGTGTGGATGAC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2045-2236 bp	1.0	79.6