Hotspot

petA-psbJ

LSC · 61929-62572 · 644 bp

Back to Sphenostylis

Pi 0.00311

MarkerSeek score 60.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 2
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	784 bp	1.0	73.7
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGCTCATCT	50.0	50.0	57.8	58.9	781 bp	1.0	71.4
petA-psbJ_p3	CGTGTCCAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.8	57.1	782 bp	1.0	71.4
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	50.0	50.0	57.8	57.1	785 bp	1.0	71.4
petA-psbJ_p5	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCAT	50.0	47.6	57.8	57.9	784 bp	1.0	69.7