Hotspot

trnQ-UUG-accD

LSC · 56296-56970 · 675 bp

Back to Sphenostylis

Pi 0.00149

MarkerSeek score 60.30

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	AGAATTGAACCACCATTTTTCCA	55.0	34.8	58.1	57.1	804-990 bp	1.0	48.8
trnQ-UUG-accD_p2	AAAATGGAAAATGGGGCGTG	AGAATTGAACCACCATTTTTCCA	45.0	34.8	57.5	57.1	863-1049 bp	1.0	45.7
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	GAATTGAACCACCATTTTTCCA	55.0	36.4	58.1	55.7	803-989 bp	1.0	45.4
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	TGAACCACCATTTTTCCATAGA	55.0	36.4	58.1	55.6	799-985 bp	1.0	44.8
trnQ-UUG-accD_p5	GAGGTTCGAATCCTTCCGTC	AGAATTGAACCACCATTTTTCCAT	55.0	33.3	58.1	57.6	804-990 bp	1.0	44.4