Hotspot

atpI-atpH

LSC · 48682-49726 · 1045 bp

Back to Sphenostylis

Pi 0.00192

MarkerSeek score 60.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1045
variable_sites: 2
pi: 0.00192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GGTGAATCCATTGAAGGCCA	CAGCAGCAATAACAGAAGCG	50.0	50.0	58.1	57.8	1111-1112 bp	1.0	77.7
atpI-atpH_p2	AGGTGAATCCATTGAAGGCC	CAGCAGCAATAACAGAAGCG	50.0	50.0	57.9	57.8	1112-1113 bp	1.0	77.7
atpI-atpH_p3	GGTGAATCCATTGAAGGCCA	GCCAATCCAGCAGCAATAAC	50.0	50.0	58.1	57.8	1118-1119 bp	1.0	77.4
atpI-atpH_p4	AGGTGAATCCATTGAAGGCC	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.9	57.8	1119-1120 bp	1.0	77.4
atpI-atpH_p5	GGTGAATCCATTGAAGGCCA	CAATCCCCTCTACAGCTTGC	50.0	55.0	58.1	58.3	1189-1190 bp	1.0	76.5