Hotspot

petN-trnC-GCA

LSC · 34399-35106 · 708 bp

Back to Sphenostylis

Pi 0.00427
MarkerSeek score 58.00
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
708
variable_sites
3
pi
0.00427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 TTAAAGCAGCCCAAGCAAGA TTTCCCCAGTTCAAATCCGG 45.0 50.0 58.0 58.1 766-771 bp 1.0 80.1
petN-trnC-GCA_p2 CAGCCCAAGCAAGACTTACT TTTCCCCAGTTCAAATCCGG 50.0 50.0 57.8 58.1 760-765 bp 1.0 78.7
petN-trnC-GCA_p3 TTAAAGCAGCCCAAGCAAGA GGGGGACTGCAAATCCTTTT 45.0 50.0 58.0 58.4 784-789 bp 1.0 77.9
petN-trnC-GCA_p4 TCTTCCCCATACTACGAGGG TTTCCCCAGTTCAAATCCGG 55.0 50.0 57.6 58.1 810-815 bp 1.0 77.1
petN-trnC-GCA_p5 CAGCCCAAGCAAGACTTACT GGGGGACTGCAAATCCTTTT 50.0 50.0 57.8 58.4 778-783 bp 1.0 76.4