Hotspot

psbD-trnT-GGU

LSC · 31028-32023 · 996 bp

Back to Sphenostylis

Pi 0.00201

MarkerSeek score 60.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 996
variable_sites: 2
pi: 0.00201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CCGCAACCAGTCATCCATAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1125-1126 bp	1.0	79.5
psbD-trnT-GGU_p2	CCGCAACCAGTCATCCATAA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1128-1129 bp	1.0	79.3
psbD-trnT-GGU_p3	CCAACCCACAAAAACGAACC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.1	58.1	1152-1153 bp	1.0	79.2
psbD-trnT-GGU_p4	CCAACCCACAAAAACGAACC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.1	58.1	1155-1156 bp	1.0	79.0
psbD-trnT-GGU_p5	AAAAACGAACCGGTCTCTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.1	58.1	1143-1144 bp	1.0	78.8