Hotspot

trnG-GCC-psbZ

LSC · 27122-27761 · 640 bp

Back to Sphenostylis

Pi 0.00625
MarkerSeek score 62.30
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
640
variable_sites
4
pi
0.00625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCA 50.0 50.0 57.8 56.8 794 bp 1.0 69.2
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGAAGGTTGGTCAA 50.0 47.6 57.8 57.5 794 bp 1.0 67.1
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TCTCCTGAAGGTTGGTCAAA 50.0 45.0 57.8 56.3 792 bp 1.0 65.3
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCAA 50.0 45.0 57.8 56.3 793 bp 1.0 65.3
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA TTCTCCTGAAGGTTGGTCAAA 50.0 42.9 57.8 57.1 793 bp 1.0 63.5