Hotspot

ycf2

Genome · 55448-62608 · 7161 bp

Back to Sphagnum

Pi 0.01

MarkerSeek score 43.00

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7161
variable_sites: 230
pi: 0.01
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GTTGGATTCACCCCGGTTTA	TCACGGACGTGTGAACAATT	50.0	45.0	57.8	58.1	745 bp	1.0	80.7
ycf2_p2	GTTGGATTCACCCCGGTTTA	CTCCTTCTTTTGGGAGGAGC	50.0	55.0	57.8	57.9	647 bp	1.0	80.1
ycf2_p3	GTTGGATTCACCCCGGTTTA	GGGGTTCACAATGGTGGAAA	50.0	50.0	57.8	58.3	515 bp	1.0	78.9
ycf2_p4	AGAGGTAGACCCCAAACGAT	TCACGGACGTGTGAACAATT	50.0	45.0	57.8	58.1	1179-1197 bp	0.8	72.3
ycf2_p5	AGAGGTAGACCCCAAACGAT	CTCCTTCTTTTGGGAGGAGC	50.0	55.0	57.8	57.9	1081-1099 bp	0.8	71.9