Hotspot

trnP-GGG-rpl32

Genome · 124354-125396 · 1043 bp

Back to Sphagnum

Pi 0.0171

MarkerSeek score 62.70

Primer available yes

Label trnP-GGG-rpl32

Feature Metadata

label_name: trnP-GGG-rpl32
feature_type: igs
region: Genome
length_bp: 1043
variable_sites: 52
pi: 0.0171
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-rpl32_p1	TAGTTTGGTAGCGTGCCATC	TGACTGGTCGTTCAAAGAGT	50.0	45.0	58.0	56.7	1176-1205 bp	1.0	77.2
trnP-GGG-rpl32_p2	AATGAAACGGAGCATAGCGT	TGACTGGTCGTTCAAAGAGT	45.0	45.0	58.0	56.7	1195-1224 bp	1.0	77.1
trnP-GGG-rpl32_p3	ACGGAGCATAGCGTAGTTTG	TGACTGGTCGTTCAAAGAGT	50.0	45.0	58.1	56.7	1189-1218 bp	1.0	76.7
trnP-GGG-rpl32_p4	TGAAACGGAGCATAGCGTAG	TGACTGGTCGTTCAAAGAGT	50.0	45.0	57.8	56.7	1193-1222 bp	1.0	75.7
trnP-GGG-rpl32_p5	GAAACGGAGCATAGCGTAGT	TGACTGGTCGTTCAAAGAGT	50.0	45.0	57.8	56.7	1192-1221 bp	1.0	75.7