Hotspot

trnL-CAA-ndhB

Genome · 92455-92777 · 323 bp

Back to Sphagnum

Pi 0.0446

MarkerSeek score 73.60

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 323
variable_sites: 47
pi: 0.0446
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CCGTTGCCTTCCTTATCGAA	50.0	50.0	58.0	58.0	496-534 bp	1.0	90.9
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CCGTTGCCTTCCTTATCGAA	50.0	50.0	58.0	58.0	494-532 bp	1.0	90.9
trnL-CAA-ndhB_p3	GTCTCGCGTGTCTACCATTT	AGGAACCTTTGCTTGCATCA	50.0	45.0	58.0	58.0	824-862 bp	1.0	87.2
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	AGGAACCTTTGCTTGCATCA	50.0	45.0	58.0	58.0	822-860 bp	1.0	87.2
trnL-CAA-ndhB_p5	TATCTGGATCCGGCTCCATT	AGGAACCTTTGCTTGCATCA	50.0	45.0	58.0	58.0	1112-1149 bp	1.0	85.9